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cd19 pe vio  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd19 pe vio
    Cd19 Pe Vio, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd19+pe/CD19+Antibody%2C+anti-human%2C+REAfinity/us12698315-681-69-70
    Average 95 stars, based on 152 article reviews
    cd19 pe vio - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: CRISPR-mediated generation of a tumor-associated antigen-deficient Raji platform to investigate antigen loss in CAR-T cell therapy
    Article Snippet: .. For CD19 and CD22 staining, cells were incubated with CD19-PE (Miltenyi Biotec) and CD22-APC (Miltenyi Biotec) antibodies for 30 min at 4 °C in the dark. .. For CD20 staining, cells were first incubated with Rabbit anti-CD20 (Abcam) for 30 min at 4 °C, washed, and then stained with a FITC-conjugated secondary antibody (Abcam) for 30 min at 4 °C in the dark.

    Article Title: A T-cell receptor targeting RUNX1 frameshift mutations in acute myeloid leukemia.
    Article Snippet: Isolated cells binding to PE-conjugated peptide-HLA (pHLA)-A- or pHLA-B-tetramers were stained with CD8-AlexaFluor 700 (Invitrogen, Carlsbad, California USA /MHCD0829), CD4-FITC (BD, Franklin Lakes, New Jersey, USA/555346), CD14-FITC (BD/555397) and CD19-FITC (BD/555412). .. Isolated cells binding to APC-conjugated pHLA-C-tetramers were stained with CD8-PacificBlue (BD/558207), CD4-PE (BD/555347), CD14-PE (BD/345785), CD19-PE (BD/ Leukemia 555413), CD158b-PE (Miltenyi Biotec/130-099-397) and an irrelevant PEconjugated pHLA-C*07:02-tetramer. .. CD158b-positive cells and cells binding to the irrelevant pHLA-C*07:02-tetramer were excluded to eliminate killercell immunoglobulin-like receptor-positive cells [37, 38]. pMHC-tetramerpositive CD8 cells that were negative for all other markers were single-cell sorted in 96-well plates by a BD FACSAria-III (BD) (Supplementary Fig. 3).

    Article Title: Establishment of an intragastric surgical model using C57BL/6 mice to study the vaccine efficacy of OMV-based immunogens against Helicobacter pylori
    Article Snippet: .. Splenocytes were stained with anti-Mabs: CD4-phycoerithrin (PE), CD8 PE, CD19 PE or an isotype control PE (Miltenyi Biotec, USA). ..

    Article Title: CRISPR-mediated generation of a tumor-associated antigen-deficient Raji platform to investigate antigen loss in CAR-T cell therapy
    Article Snippet: .. For CD19 and CD22 staining, cells were incubated with CD19-PE (Miltenyi Biotec) and CD22-APC (Miltenyi Biotec) overnight at 4 °C in the dark. .. For CD20 staining, cells were incubated with Rabbit anti-CD20 (Abcam) overnight at 4 °C, washed with PBS, and then incubated with a FITC-conjugated secondary antibody for 1 h at RT in the dark.

    Incubation:

    Article Title: CRISPR-mediated generation of a tumor-associated antigen-deficient Raji platform to investigate antigen loss in CAR-T cell therapy
    Article Snippet: .. For CD19 and CD22 staining, cells were incubated with CD19-PE (Miltenyi Biotec) and CD22-APC (Miltenyi Biotec) antibodies for 30 min at 4 °C in the dark. .. For CD20 staining, cells were first incubated with Rabbit anti-CD20 (Abcam) for 30 min at 4 °C, washed, and then stained with a FITC-conjugated secondary antibody (Abcam) for 30 min at 4 °C in the dark.

    Article Title: CRISPR-mediated generation of a tumor-associated antigen-deficient Raji platform to investigate antigen loss in CAR-T cell therapy
    Article Snippet: .. For CD19 and CD22 staining, cells were incubated with CD19-PE (Miltenyi Biotec) and CD22-APC (Miltenyi Biotec) overnight at 4 °C in the dark. .. For CD20 staining, cells were incubated with Rabbit anti-CD20 (Abcam) overnight at 4 °C, washed with PBS, and then incubated with a FITC-conjugated secondary antibody for 1 h at RT in the dark.

    Immunopeptidomics:

    Article Title: Depletion of Neonatal Neutrophilic Cells Worsens the Outcome of E. coli Sepsis in Newborn Mice
    Article Snippet: .. Antibodies were purchased from BD Biosciences (CD3 FITC [145‐2C11], CD4 APC [RM4‐5], CD11b FITC [M1/70], CD45 PerCp [30‐F11], TLR4 [CD284/MD‐2] PE [MTS510] FSV510 [Amcyan], from BioLegend CD4 APC‐Cy7 [GK1.5], CD19 PE [1D3], CD25 APC [PC61] Gr‐1 PE‐Cy7 [RB6‐8C5], Ly6G APC [1A8], NKp46 PE‐Cy7 [29A1.4], CD11c BV421 [N418], F4/80 APC [BM8] MHC II APC‐Cy7 [M5/114.15.2], 7‐amino‐actinomycin D [7AAD], Annexin V [PE/Cyanine7] or Miltenyi Biotec Ly‐6G FITC [REA526]). ..

    Article Title: Depletion of Neonatal Neutrophilic Cells Worsens the Outcome of E. coli Sepsis in Newborn Mice.
    Article Snippet: .. Antibodies were purchased from BD Biosciences (CD3 FITC [145-2C11], CD4 APC [RM4-5], CD11b FITC [M1/70], CD45 PerCp [30-F11], TLR4 [CD284/MD-2] PE [MTS510] FSV510 [Amcyan], from BioLegend CD4 APC-Cy7 [GK1.5], CD19 PE [1D3], CD25 APC [PC61] Gr-1 PE-Cy7 [RB6-8C5], Ly6G APC [1A8], NKp46 PE-Cy7 [29A1.4], CD11c BV421 [N418], F4/80 APC [BM8] MHC II APC-Cy7 [M5/114.15.2], 7-amino-actinomycin D [7AAD], Annexin V [PE/Cyanine7] or Miltenyi Biotec Ly-6G FITC [REA526]). ..

    Isolation:

    Article Title: A T-cell receptor targeting RUNX1 frameshift mutations in acute myeloid leukemia.
    Article Snippet: Isolated cells binding to PE-conjugated peptide-HLA (pHLA)-A- or pHLA-B-tetramers were stained with CD8-AlexaFluor 700 (Invitrogen, Carlsbad, California USA /MHCD0829), CD4-FITC (BD, Franklin Lakes, New Jersey, USA/555346), CD14-FITC (BD/555397) and CD19-FITC (BD/555412). .. Isolated cells binding to APC-conjugated pHLA-C-tetramers were stained with CD8-PacificBlue (BD/558207), CD4-PE (BD/555347), CD14-PE (BD/345785), CD19-PE (BD/ Leukemia 555413), CD158b-PE (Miltenyi Biotec/130-099-397) and an irrelevant PEconjugated pHLA-C*07:02-tetramer. .. CD158b-positive cells and cells binding to the irrelevant pHLA-C*07:02-tetramer were excluded to eliminate killercell immunoglobulin-like receptor-positive cells [37, 38]. pMHC-tetramerpositive CD8 cells that were negative for all other markers were single-cell sorted in 96-well plates by a BD FACSAria-III (BD) (Supplementary Fig. 3).

    Binding Assay:

    Article Title: A T-cell receptor targeting RUNX1 frameshift mutations in acute myeloid leukemia.
    Article Snippet: Isolated cells binding to PE-conjugated peptide-HLA (pHLA)-A- or pHLA-B-tetramers were stained with CD8-AlexaFluor 700 (Invitrogen, Carlsbad, California USA /MHCD0829), CD4-FITC (BD, Franklin Lakes, New Jersey, USA/555346), CD14-FITC (BD/555397) and CD19-FITC (BD/555412). .. Isolated cells binding to APC-conjugated pHLA-C-tetramers were stained with CD8-PacificBlue (BD/558207), CD4-PE (BD/555347), CD14-PE (BD/345785), CD19-PE (BD/ Leukemia 555413), CD158b-PE (Miltenyi Biotec/130-099-397) and an irrelevant PEconjugated pHLA-C*07:02-tetramer. .. CD158b-positive cells and cells binding to the irrelevant pHLA-C*07:02-tetramer were excluded to eliminate killercell immunoglobulin-like receptor-positive cells [37, 38]. pMHC-tetramerpositive CD8 cells that were negative for all other markers were single-cell sorted in 96-well plates by a BD FACSAria-III (BD) (Supplementary Fig. 3).

    Control:

    Article Title: Establishment of an intragastric surgical model using C57BL/6 mice to study the vaccine efficacy of OMV-based immunogens against Helicobacter pylori
    Article Snippet: .. Splenocytes were stained with anti-Mabs: CD4-phycoerithrin (PE), CD8 PE, CD19 PE or an isotype control PE (Miltenyi Biotec, USA). ..



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