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pe cd19 antibody  (Miltenyi Biotec)


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    Miltenyi Biotec pe cd19 antibody
    (A) Gating strategy for sorting singlets of B and T cells. (B) Sorted counts. Leukocytes were isolated from previously frozen blood clots and dissociated by following section A of this protocol. Cells were stained with an antibody panel targeting CD3, <t>CD19,</t> CD11b, and CD11c. Viability marker was not used due to interest in nuclei from subpopulations of non-viable leukocytes. After doublet exclusion and gating on height and area of cells, T-cell subsets were identified as CD3+ populations. B-cell subsets were identified as CD19+ populations. CD11b and CD11c were used to exclude monocytes. Single-stain controls were used for compensation. During this sort, we successfully sorted 57,894 T cells and 24,648 B cells. The full report is available in Supplementary material (Report S1).
    Pe Cd19 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe cd19 antibody/product/Miltenyi Biotec
    Average 95 stars, based on 316 article reviews
    pe cd19 antibody - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "Nuclei Isolation Methods on Frozen Clotted Blood Samples"

    Article Title: Nuclei Isolation Methods on Frozen Clotted Blood Samples

    Journal: Bio-protocol

    doi: 10.21769/BioProtoc.5573

    (A) Gating strategy for sorting singlets of B and T cells. (B) Sorted counts. Leukocytes were isolated from previously frozen blood clots and dissociated by following section A of this protocol. Cells were stained with an antibody panel targeting CD3, CD19, CD11b, and CD11c. Viability marker was not used due to interest in nuclei from subpopulations of non-viable leukocytes. After doublet exclusion and gating on height and area of cells, T-cell subsets were identified as CD3+ populations. B-cell subsets were identified as CD19+ populations. CD11b and CD11c were used to exclude monocytes. Single-stain controls were used for compensation. During this sort, we successfully sorted 57,894 T cells and 24,648 B cells. The full report is available in Supplementary material (Report S1).
    Figure Legend Snippet: (A) Gating strategy for sorting singlets of B and T cells. (B) Sorted counts. Leukocytes were isolated from previously frozen blood clots and dissociated by following section A of this protocol. Cells were stained with an antibody panel targeting CD3, CD19, CD11b, and CD11c. Viability marker was not used due to interest in nuclei from subpopulations of non-viable leukocytes. After doublet exclusion and gating on height and area of cells, T-cell subsets were identified as CD3+ populations. B-cell subsets were identified as CD19+ populations. CD11b and CD11c were used to exclude monocytes. Single-stain controls were used for compensation. During this sort, we successfully sorted 57,894 T cells and 24,648 B cells. The full report is available in Supplementary material (Report S1).

    Techniques Used: Isolation, Staining, Marker

    Read depths are generous at the start site of beta actin, indicative of an accessible chromatin region for both CD3+ T cells and CD19+ B cells. For CD3E, an accessible chromatin region is present in T cells with more read depth, but not in B cells. CD79A is a known marker in B cells and has generous read depths for B cells but is absent in T cells.
    Figure Legend Snippet: Read depths are generous at the start site of beta actin, indicative of an accessible chromatin region for both CD3+ T cells and CD19+ B cells. For CD3E, an accessible chromatin region is present in T cells with more read depth, but not in B cells. CD79A is a known marker in B cells and has generous read depths for B cells but is absent in T cells.

    Techniques Used: Marker



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    Image Search Results


    (A) Gating strategy for sorting singlets of B and T cells. (B) Sorted counts. Leukocytes were isolated from previously frozen blood clots and dissociated by following section A of this protocol. Cells were stained with an antibody panel targeting CD3, CD19, CD11b, and CD11c. Viability marker was not used due to interest in nuclei from subpopulations of non-viable leukocytes. After doublet exclusion and gating on height and area of cells, T-cell subsets were identified as CD3+ populations. B-cell subsets were identified as CD19+ populations. CD11b and CD11c were used to exclude monocytes. Single-stain controls were used for compensation. During this sort, we successfully sorted 57,894 T cells and 24,648 B cells. The full report is available in Supplementary material (Report S1).

    Journal: Bio-protocol

    Article Title: Nuclei Isolation Methods on Frozen Clotted Blood Samples

    doi: 10.21769/BioProtoc.5573

    Figure Lengend Snippet: (A) Gating strategy for sorting singlets of B and T cells. (B) Sorted counts. Leukocytes were isolated from previously frozen blood clots and dissociated by following section A of this protocol. Cells were stained with an antibody panel targeting CD3, CD19, CD11b, and CD11c. Viability marker was not used due to interest in nuclei from subpopulations of non-viable leukocytes. After doublet exclusion and gating on height and area of cells, T-cell subsets were identified as CD3+ populations. B-cell subsets were identified as CD19+ populations. CD11b and CD11c were used to exclude monocytes. Single-stain controls were used for compensation. During this sort, we successfully sorted 57,894 T cells and 24,648 B cells. The full report is available in Supplementary material (Report S1).

    Article Snippet: Flow cytometry antibodies (only applicable if flow sorting) a. FITC CD3 antibody (Miltenyi Biotec, catalog number: 130-113-700) b. PE CD19 antibody (Miltenyi Biotec, catalog number: 130-114-172) c. VioBlue CD11B (Miltenyi Biotec, catalog number: 130-110-616) d. APC CD11C (Miltenyi Biotec, catalog number: 130-114-110) e. Other antibodies (optional): Add other suitable antibodies to tailor the cell population of interest 3.

    Techniques: Isolation, Staining, Marker

    Read depths are generous at the start site of beta actin, indicative of an accessible chromatin region for both CD3+ T cells and CD19+ B cells. For CD3E, an accessible chromatin region is present in T cells with more read depth, but not in B cells. CD79A is a known marker in B cells and has generous read depths for B cells but is absent in T cells.

    Journal: Bio-protocol

    Article Title: Nuclei Isolation Methods on Frozen Clotted Blood Samples

    doi: 10.21769/BioProtoc.5573

    Figure Lengend Snippet: Read depths are generous at the start site of beta actin, indicative of an accessible chromatin region for both CD3+ T cells and CD19+ B cells. For CD3E, an accessible chromatin region is present in T cells with more read depth, but not in B cells. CD79A is a known marker in B cells and has generous read depths for B cells but is absent in T cells.

    Article Snippet: Flow cytometry antibodies (only applicable if flow sorting) a. FITC CD3 antibody (Miltenyi Biotec, catalog number: 130-113-700) b. PE CD19 antibody (Miltenyi Biotec, catalog number: 130-114-172) c. VioBlue CD11B (Miltenyi Biotec, catalog number: 130-110-616) d. APC CD11C (Miltenyi Biotec, catalog number: 130-114-110) e. Other antibodies (optional): Add other suitable antibodies to tailor the cell population of interest 3.

    Techniques: Marker